Nitrotyrosine Monoclonal Antibody (Clone CC.22.8C7.3) 189542 from Cayman Chemical

Product Specs
ItemNitrotyrosine Monoclonal Antibody (Clone CC.22.8C7.3)
CompanyCayman Chemical
Price Pricing Info Supplier Page View Company Product Page
Catalog Number189542
Quantity200 µg
ApplicationsELISA, Western Blot
FormatPBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
IsotypeIgG2b
CloneCC.22.8C7.3
HostMouse
ImmunogenPeroxynitrite-treated KLH
ClonalityMonoclonal
Research AreaAcute Phase Reactants, Alzheimer’s Disease, Atherosclerosis, Autoimmunity, Blood, Cancer, Cardiovascular Diseases, Cardiovascular System, Gastric Disease, Immunology & Inflammation, Lipids & Lipoproteins, Lipoproteins, Myeloperoxidase, Neurodegenerative Disorders, Neuroscience, Oxidative Stress & Reactive Species, Parkinson’s Disease, Protein Oxidation, Reactive Nitrogen, Reactive Oxygen, Rheumatoid Arthritis, Serum Proteins, Ulcerative Colitis
Antibody TypePrimary
Purity50 µg or 200 µg of protein G-purified antibody

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Ulva pertusa, a Marine Green Alga, Attenuates DNBS-Induced Colitis Damage via NF-κB/Nrf2/SIRT1 Signaling PathwaysJournal of Clinical MedicineJuly 25, 2022Alessio Ardizzone, Alessia Filippone, Deborah Mannino, Sarah Adriana Scuderi, Giovanna Casili, Marika Lanza, Laura Cucinotta, Michela Campolo, Emanuela Espositoanti-COX-2 (Santa Cruz Biotechnology sc-376861, 1:100 in PBS, v/v), or anti-Nitrotyrosine (Cayman #189542, 1:100 in PBS, v/v). At the end of the overnight incubation with the primary antibody, slides were washed

(2) Nitrate consumption preserves HFD-induced skeletal muscle mitochondrial ADP sensitivity and lysine acetylation: A potential role for SIRT1Redox BiologyJune 1, 2022Henver S. Brunetta, Heather L. Petrick, Iman Momken, Rachel M. Handy, Christopher Pignanelli, Everson A. Nunes, Jérôme Piquereau, Mathias Mericskay, Graham P. Holloway1:1000), 4-HNE (Alpha Diagnostics cat. no. HNE11-S, 1:1000), Nitrotyrosine (Cayman Chemicals cat. no. 189542, 1:500), alpha-tubulin (1:5000, Abcam cat. no. ab7291, Eugene, OR, USA). All membranes were detected

(3) Heterozygous SOD2 deletion selectively impairs SERCA function in the soleus of female micePhysiological reportsMay 1, 2022Braun JL, Messner HN, Cleverdon REG, Baranowski RW, Hamstra SI, Geromella MS, Stuart JA, Fajardo VA.µL eluent LV: 10,BioRad PreCast TGX 4–15% gradient gels,PVDF (homogenate) and nitrocellulose (IP),"#189542, Cayman Chemical" Western blotting specific details. Details regarding the protein load, electrophoresis,

(4) Characterizing SERCA Function in Murine Skeletal Muscles after 35-37 Days of SpaceflightInternational journal of molecular sciencesOctober 29, 2021Braun JL, Geromella MS, Hamstra SI, Messner HN, Fajardo VA.from Proteintech (26905-1-AP, Rosemont, IL, USA). Nitrotyrosine was purchased from Cayman Chemical (189542, Ann Arbor, MI, USA), and nitrocysteine was obtained from Abcam (ab94930, Cambridge, UK). Finally, the

(5) Western Diet Causes Obesity-Induced Nonalcoholic Fatty Liver Disease Development by Differentially Compromising the Autophagic ResponseAntioxidants (Basel)October 15, 2020Ines C. M. Simoes et al.Sigma-Aldrich) in tissue homogenate. The nitration of proteins was analyzed using a nitrotyrosine antibody (189542, Cayman Chemical, Ann Arbor, MI, USA). Briefly, 50 µg of mitochondrial fraction and total liver homogenate

(6) Preconditioning but not postconditioning treatment with resveratrol substantially ameliorates post‑resuscitation myocardial dysfunction through the PI3K/Akt signaling pathwayMolecular Medicine ReportsMay 31, 2019Haihong Zhang, Qinqin Wu, Zhi Wan, Yu Cao, Zhi Zengendogenous peroxidase activity. The sections were then incubated with anti-nitrotyrosine (1:1,000; 189542-50; Cayman Chemical) antibody at 4°C overnight in a moist chamber. Goat anti-mouse Biotinylated secondary

(7) Attenuation of oxidative stress-induced lesions in skeletal muscle in a mouse model of obesity-independent hyperlipidaemia and atherosclerosis through the inhibition of Nox2 activityFree radical biology & medicineDecember 1, 2018Sfyri PP, Yuldasheva NY, Tzimou A, Giallourou N, Crispi V, Aburima A, Beltran-Alvarez P, Patel K, Mougios V, Swann JR, et al.anti-mouse Species Mouse Mouse Mouse Mouse Mouse Goat Dilution 1:200 1:1,000 1:1000 1:800 1:1,000 1:10,000 55 Supplier, Cat Number Cayman 189542 R&D Systems MAB3249 Santa Cruz 166182 Santa Cruz 7972 EMD Millipore 05-661 Sigma A9044 Supplementary

(8) Activation of mosquito complement antiplasmodial response requires cellular immunityScience ImmunologyJuly 25, 2017Julio César Castillo, Ana Beatriz Barletta Ferreira, Nathanie Trisnadi, Carolina Barillas-Mury(1:2000) (Abcam, rabbit anti-GFP, Ab290) and mouse α-nitrotyrosine (1:150) (Cayman Chemical, item # 189542). Midguts were then washed three times in PBT-Gelatin and incubated with the secondary antibodies donkey

(9) Diaphragm Abnormalities in Patients with End-Stage Heart Failure: NADPH Oxidase Upregulation and Protein OxidationFrontiers in PhysiologyJanuary 9, 2017Bumsoo Ahn, Philip D. Coblentz, Adam W. Beharry, Nikhil Patel, Andrew R. Judge, Jennifer. S. Moylan, Charles W. Hoopes, Mark R. Bonnell, Leonardo F. FerreiraCarbonyl Immunoblot kit, Cell Biolabs), 4-hydroxynonenal (4-HNE, Ab46545, AbCam) adducts, and 3-nitrotyrosines (3-NT, 189542, Cayman Chemical). To probe for sources of ROS, we used primary antibodies targeting Nox2 (CYBB, 1:500

(10) Preservation of Renal Blood Flow by the Antioxidant EUK-134 in LPS-Treated PigsInternational Journal of Molecular SciencesMarch 25, 2015Sheldon Magder, Dimitrios G. Parthenis, Imad Al Al Ghoulehh, or overnight, at 4 °C. Nitrotyrosine was detected with anti-nitrotyrosine monoclonal antibody (Cat # 189542, Cayman Chemical Co., Ann Arbor, MI, USA). The membranes were washed three times with wash buffer and

(11) Oxidative/Nitrative Stress and Inflammation Drive Progression of Doxorubicin-Induced Renal Fibrosis in Rats as Revealed by Comparing a Normal and a Fibrosis-Resistant Rat StrainPloS oneJanuary 1, 2015Szalay CI, Erdélyi K, Kökény G, Lajtár E, Godó M, Révész C, Kaucsár T, Kiss N, Sárközy M, Csont T, et al.and immunohistochemistry: 4-hydroxy-2-nonenal (HNE, mouse monoclonal, clone: HNEJ-2, JaICA, Japan), NT (mouse monoclonal, #189542, Cayman Chemical Company, Michigan, IL), fibronectin (rabbit polyclonal, Sigma-Aldrich, Budapest, Hungary),

(12) Sulforaphane Attenuates Gentamicin-Induced Nephrotoxicity: Role of Mitochondrial ProtectionEvidence-Based Complementary and Alternative MedicineApril 4, 2013Mario Negrette-Guzmán, Sara Huerta-Yepez, Omar Noel Medina-Campos, Zyanya Lucía Zatarain-Barrón, Rogelio Hernández-Pando, Ismael Torres, Edilia Tapia, José Pedraza-ChaverriMolecular Probes, Inc. (Eugene, OR, USA). Mouse monoclonal anti-nitrotyrosine (3-NT) antibody (Cat. no. 189542) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Mouse IgG2a κ isotype control (Cat. no. 550339)

(13) Exercise restores endothelial function independently of weight loss or hyperglycaemic status in db/db miceDiabetologiaJuly 1, 2008F Moien-Afshari, S Ghosh, S Elmi, M M Rahman, N Sallam, M Khazaei, T J Kieffer, R W Brownsey, I LaherDiego, CA, USA]), sheep (Cu/Zn-SOD or MnSOD; #574597, #574596; Calbiochem) or (2) mouse (nitrotyrosine; #189542; Cayman Chemicals). Following triple washes in TBS-T, membranes were incubated for 2 h at room temperature

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Nitrotyrosine Monoclonal Antibody (Clone CC.22.8C7.3) from Cayman Chemical

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Cayman Chemical

Citations (13)

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Nitrotyrosine Monoclonal Antibody (Clone CC.22.8C7.3) from Cayman Chemical

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